LIFE SCIENCE DISCUSSIONS

Discussion of anything scientific that does not fit into another category or forum. Examples range from “how to write the statistical symbol for mean” to “my TC incubator is contaminated.”

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Discussion of grants, funding, science jobs, manuscript preparation and submission, statistics, and business opportunity

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Discussion chromatography (HPLC, FPLC, GC) spectroscopy, surface chemistry, thermal analysis, sample separation, extraction

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Discuss organization of the body and its functions including organs, tissues, electrophysiology, histology, fixation, immunohistochemistry, exercise, muscle, microdissection…

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Discussion about breeding, diseases, genetics, management, ethics, nutrition and physiology of farm, laboratory, companion animals…

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Discussion on antibodies techniques including polyclonal, monoclonal, peptide design, antisera, labeling, ELISA, fusion proteins, complement activation, isotypes, Fab, Fc, class switching…

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Discussion about chemical processes in living organisms. Including enzymes, kinetics, biosynthesis, catalysis, nutrient metabolism, glycolysis, amino acid synthesis…

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Discussion on issues in bioethics including medical bioethics, ethics in the laboratory, plagiary, euthanasia, cloning, artificial life, organ donation, stem cell use…

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Discussion bioinformatics including software development, programming, systems biology, computational models, semantic web, ontologies, data extraction, gene annotation…

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Discussion on medical devices intended to interact with biological systems. Examples joint replacement, bone plates, cement, dental implants, heart valves, lens…

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Interdisciplinary field applying techniques from the physical sciences to understanding biological structure and function at the molecular level.

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Discussion on 2D and 3D cell and tissue culture, primary cells, cell lines and transformed cells, isolation and culture of particular cell types such as stem cells, lymphocytes, epithelial and endothelial cells, transfection, electroporation, cell imaging, detachment, apoptosis…

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Discussion on biochemical reactions inside the cell transmitting signals involving hormones, neurotransmitters, cytokines, growth factors, kinases, phosphotases, second messengers, transcription factors, protein and target genes…

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Discussion on clinical research topics including trials, data management, monitoring, publishing and impact.

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Discussion on the various techniques of cytology and histology including image analysis, pathology, slides, stains, and immunohistochemistry.

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Discussion of allele-specific mutation detection, diagnostic imaging, Pyrosequencing, Molecular Cytogenetics, Genetic Testing, Forensics, paternity testing. novel biomarkers, personalized medicine...

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Discussion of anything relating to DNA including structure, sequence, replication purification, PCR, detection, and DNA binding proteins.

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Discussion of recombinant gene expression including, analysis, cloning, plasmids, and transformation.

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Discussion of various gene silencing mechanisms and techniques, both in vitro and in vivo, including miRNA, RNAi, shRNA, antisense and morpholinos.

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Discussion of the broad topic of genetics, ranging from gene expression, gene therapy, and genetic engineering to transgenics…

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Discussion on methodology for high-throughput genome scale analysis including next-generation sequencing, gene expression, SNP genotyping, CGH, bioinformatic approaches and useful software for genomic testing…

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Discussion of the Immune System including T cells, B cells, macrophages, Natural Killer T cells, bone marrow, chemokines, cytokines, innate immunity, blood types, immune response, inflammation…

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Discussion on novel techniques in automating laboratory techniques. Including microplate systems, liquid handling and dispensing systems, data management, robotics, total laboratory automation systems...

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Discussion on single cell organisms including gram-negative/gram positive bacteria, yeast, parasites, operons, algae, protozoa, plating, colony formation, media, antibiotics, glycocalyx, lipopolysaccharide (LPS), endotoxin, replica plating, Fungi

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Discussion on scanning and transmission electron microscopy (SEM), atomic force microscopy (AFM), confocal, lenses, microscope brands, in vivo imaging, fluorescence, 2D rendering, imaging software, FRET, CT, MRI..

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Discussion on the use of model organisms in research including, yeast, C. elegans, Drosophila, zebrafish, Xenopus, mice/rats, and higher mammals.

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Discussions on important issues surrounding nanoscience and emerging nanotechnology; colloid science, quantum dots, molecular self assembly, and synthetic molecular motors, nanotubes . . .

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Discussion of all matters and experiments covering neural transmission, molecular neuroscience, systems neuroscience, neurodegeneration, learning and memory, behavior, axons, neurons…

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Discussion of organic chemical reactions including reagents, protecting groups, oxidation, reducing agents, deprotection, techniques, equipment, reaction details, substitutions, bonds, IUPAC, chirality, stereochemistry,

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Discussion on drug interaction with living organisms, drug composition and properties, toxicology, therapy, medical applications, pharmacokinetics…

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Discussion on Western blotting, SDS-PAGE gels, chromatography, glycosylation, O-GlcNAc, phosphorylation, ubiquitination, protein folding, crystallography, denaturization…

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Discussion of the various techniques used for protein detection including electrophoresis, westerns, labeling, elisa, and chromatography.

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Discussion on the proteome, 2D Gels, spot analysis, mass spectrometry, MudPIT, electrospray, LC/MS/MS, analysis softeware, peptide identification, phophopeptides, quantitation…

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Discussion about RNA related topics including isolation, extraction, transcription, splicing, translation and library construction.

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Discussion of stem cell related topics including adult, embryonic, pluripotent, and multipotent stem cells, iPS, isolation, cell culture, and differentiation

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Discussion on all things viral including phage, DNA virus, RNA virus, entry, envelope, retrovirus, fusion, plaque formation, vaccines, emerging viral diseases…

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