In my experiment I have to prepare some reagents that have not been contaminated by ATP. I need the pure ADP solution, therefore, I used Lr/L reagent in the ATP assay (Promega) to check the ATP contamination in both of water and ADP solution.
I used the Luminometer to measure the presence of ATP in ADP solution. The control is only water. Unfortunately, the value got from the sample containing ADP solution is very high comparing to the control. I don’t know why the ADP solution containing ATP? because the water used as the solvent of ADP was not contaminated by ATP. And the ADP solution making condition is not ATP-contaminated.
Could any one help me explain why in the ADP solution (using water as a solvent), the ATP component is appeared?
Thank a lot!