calculation of rrf

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desaraju_nadh
desaraju_nadh's picture
calculation of rrf

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 How to rrf calculation in hplc

Ajay Kumar
Ajay Kumar's picture
u can find the formula in US

u can find the formula in US Pharmacopoeia/ Indian Pharmacopoeia 

Ajay Kumar
Ajay Kumar's picture
Response Factor = [response

Response Factor = [response factor of individual related substance/ response factor of drug substance] 

If a linearity curve is constructed for both the related substance and the drug substance by plotting the response versus the concentration, the relative response factor can also be determined by
Relative response     factor=                      
 
sloperelatedsubstance / slopedrugsubstance                                                                          
 Plz check this website also: Page no 41

http://bib.tiera.ru/DD2C2E92-F7D3-49B4-A0A3-A418AE40BC78/FinalDownload/DownloadId-136ED942AC2D76E5887D831A5D158EFA/DD2C2E92-F7D3-49B4-A0A3-A418AE40BC78/dvd38/Chan%20C.C.,%20Lee%20Y.C.,%20Lam%20H.%20-%20Analytical%20method%20validation%20and%20instrument%20performance%20verification(2004)(320).pdf