I am doing the analysis of sorbitol using cation exchange chromatography
the column conditions are as follows
a stainless steel column 0.3 m long and 7.8 mm in internal diameter packed with strong cation excahange resin (calcium form) ( 9µm ) and maintained at a temperature of 85 ± 1 ° C
Mobile phase is water
Now my question is
On what principle RID works? and How to decide the detector at the stage of method development (for RID)? what is the importance of temperature of the column? why so heigh end temperature?
and how the column equilbrated after elution of the sample?
Cation ion exchange chromatography