Cationexchange Column for trypitc digest (peptide map)

2 posts / 0 new
Last post
Diablo
Diablo's picture
Cationexchange Column for trypitc digest (peptide map)

Hi guys,

do you have experience with ionexchange HPLC - especially in seperation of peptides ?
Did you try several columns/buffers ?  Then you might help me.

I've got a trpytic digest of a protein on which i want to perform a peptide map.
I'm NOT looking for a seperation with reverse phase HPLC. 
I'm looking for a seperation with ionexchange-HPLC (preferably  cationexchange), but im not sure about what columns/buffers i should use.

g a
g a's picture
Use a nano bore strong Cation