do you have experience with ionexchange HPLC - especially in seperation of peptides ?
Did you try several columns/buffers ? Then you might help me.
I've got a trpytic digest of a protein on which i want to perform a peptide map.
I'm NOT looking for a seperation with reverse phase HPLC.
I'm looking for a seperation with ionexchange-HPLC (preferably cationexchange), but im not sure about what columns/buffers i should use.