I am going to analyze Trimethyl borate on GC, Initially I have used TC-1 and get RT about 5 minutes but after some time RT was reduced to 3 minutes and peak tailing was observed.
I have doubt on column and I analyze it on DB-XLB column I got good peak shape and RT near 3.5 minutes. After some time again RT shift up ward i.e 1.9 minutes and peak tailing was observed.
I donot understand what is the resion.
My column condition is 60 degree (hold 2min.)@ 10 degree up to 200degree(3min. hold)
Injetor is 200 degree and detector was 259 degree.
carrier is N2 with 10: 1 split and 10 psi flow rate/.
As I understand TMB is harmful for both column or we should try another column for the analysis.