does anyone of you know a good HPLC column for the separation of PEGylated (PEG = very hydrophobic) protein/peptids ? Im working with high molecular weight PEG (20kD).
In general im looking for a column with the ability to separate based on hydrophobicity but also based on the protein/peptide properties ... So far along i tried Acclaim's mixed mode WCX column and i'm still testing it.
Does anyone of you have an idea of what kind of columns i could else try ?