HPLC COLUMN

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amit_chem
amit_chem's picture
HPLC COLUMN

Sir,
In case of HPLC columns,what is the meaning of  BDS ,XDB ,RP,end cap.

Dr. Analytical
Dr. Analytical's picture
There are many acronyms used

There are many acronyms used in HPLC.  We cannot list them all here, but there are several groups that I can describe:

Marketing Names:
XDB, SB, RX are brand names for certain columns from Agilent Technologies.  Each phase is prepared in a different way and has different separation properties.
Each manufacturer has their own brand names, for example: XTerra (Waters), Discovery (Supelco), Ultra (Restek).
Bonded Phase:
C18, C8, Phenyl, Diol, Cyano, PFP - all describe the groups that are bonded to the surface, and therefore change the properties of the surface.
HPLC Terms:
RP=Reversed phase
NP=Normal phase
End Cap=extra bonding step on silica-based phases, to cover up any unreacted silanol groups

You should get a book on HPLC for refererence, or attend some training classes so that you can more completely understand these ideas.