HPLC Peak Shape

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Rachel Giffin
Rachel Giffin's picture
HPLC Peak Shape

I have an 1100 agilent HPLC that I just put a new column in.  I'm getting a lot of tailing on all my peaks.  I put this column in my other 1100 and got nice sharp peaks.  The solvents and methods are the same for each machine.  All the literature says peak tailing is most likely cased by the column, but don't offer a lot of solutions when it isn't the column.  Any suggestions to what is causing the tailing?  I really appreciate the help.