Hi there guys
Hope all is well
Does anyone have any idea about running conditions on Lycopene and Lutein with HPLC?
Thanks in advance
There are many published methods, including:
"A rapid method for the extraction, separation and quantification of the geometric isomers of lycopene and beta-carotene from tomato fruit is described. Carotenoids in tomato were separated and eluted using a reversed-phase HPLC with a C30 column and a mobile phase consisting of methyl-t-butyl ether, methanol and ethyl acetate. The system provided sharp resolution of cis- and trans-isomers of lycopene within approximately 23 min in contrast to the longer and more complex gradient procedures required by previously described methods. Experiments indicate that the stability of extracts of fresh tomato may be improved if stored at -20 degrees C, and that the presence of the antioxidant BHA has no apparent effect on stability."
I believe the big problem will be solubility. These compounds are not soluble in water, and probably have little solubility in either methanol or acetonitrile. So, traditional reversed phase approaches may be difficult. In the above example, they are using "non-aqueous reversed phase" conditions. If you could dissolve the materials in THF, maybe a C18 or C8 column using THF mobile phase might work, but retention time would be long.
You could also try a normal phase approach.
These guys are not soluble in Acetonitrile nor MeOH
I will try THF and keep you posted sir
Thank you very much
You always have an answer Dr.A and I want to thank you for that