Measurement of environmental contaminant level in animal tissue

11 posts / 0 new
Last post
msvnathan
msvnathan's picture
Measurement of environmental contaminant level in animal tissue

hi
i am working on pesticide effects on male reproduction. i want to measure the level of injected pesticide in the testis of male rats supernatant. how to measure using HPLC. what kind of column can be used?

Thanks in advance.

Greg Pronger
Greg Pronger's picture
Which pesticides or class of

Which pesticides or class of pesticides are you investigating?

Greg

msvnathan
msvnathan's picture
I am using organochloring

I am using organochloring like methoxychlor, lindane.

Bryan Evans
Bryan Evans's picture
What types of detector(s) do

What types of detector(s) do you have access to?
Lindane doesn't look like it has much of a chromophore.

Greg Pronger
Greg Pronger's picture
Do you have access to a GC

Do you have access to a GC with an ECD. The chlorinated pesticides respond extraordinarily well on there. I've not seen a response, so has the issue been resolved?

msvnathan
msvnathan's picture
Dear Greg,

Dear Greg,
sorry for late reply for your posts. Since i was out of station i could not reply. still that issue is in pending. i am working on organochlorine pesticides like methoxychlor, lindane. Yes, i too have access to GC with ECD. expecting ur suggestion.
thanks

Greg Pronger
Greg Pronger's picture
On the ECD, both of these

On the ECD, both of these would work very well. You'll simply need to perform a clean-up to get rid of co-extracted fats, lipids, etc (though they may not respond, they can be hard on the column).
For instrument conditions, the USEPA has a website called "National Environmental Methods Indes (NEMI). It's at:
http://www.nemi.gov/apex/f?p=237:1:3597128292230866 (link fixed)
You can type in an analyte, and if the EPA has a method, it will list all applicable, and usually, have a PDF of the method. 8081B is a GC-ECD method that would handle both, and would be a good starting point. For GC instrument conditions, not knowing what column you may have, I would go to the manufacturer and start there.
The last bit is clean-up. The FDA has a lot of procedures for cleaning up sample matrices prior to going to the instrument. They call their manual "Pesticides Analytical Methods, or "PAM" for short. Their online manual is at:
http://www.cfsan.fda.gov/~frf/pami2.html. (link fixed)
If you take a look at that and the EPA methods, you should be able to put together a procedure that handles your matrix without a problem. Particularly regarding the EPA method, for your prposes, I would necessarily simply do what they've put in place, a lot of what they have in the document is QA protocols. However, the instrument end of things should be useful and be a good starting poit when you combine it with whatever info you can get from the manufacturer of your column.
Greg
 

efoconnor
efoconnor's picture
What is the structure of the

What is the structure of the pesticide?? Is it an organophosphate?  An Arsenical?  Need to provide greater info. 

efoconnor
efoconnor's picture
Apologies:  I did not read

Apologies:  I did not read down.  If you know the class and IUPAC name, you can do a search online and find which columns and methods are used for this class of compounds.    Unless it is uniques there should be a great deal out there.  You can also go to manufactures and search applications.

Dr. Analytical
Dr. Analytical's picture
The main compounds mentioned

The main compounds mentioned were methoxychlor and lindane.  As Greg noted, there are well-established GC methods.  But if you have any new suggestions, we welcome them here.
 

msvnathan
msvnathan's picture
hi thanku so much for ur

hi thanku so much for ur replies. i will try it.
thanks