Mechanism in sepearation of enantiomers in Normal phase silica columns

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mbbschem07
mbbschem07's picture
Mechanism in sepearation of enantiomers in Normal phase silica columns

Hello everyone,
Apologies if this is a rather straightforward query, but I'm not an expert in this area. I need how to separate enantiomers in normal silica columns by HPLC and mechanism.
Please give me suggestions to my question
Thanks to all
NBK Rao

Sami Tuomivaara
Sami Tuomivaara's picture
mbbschem07,

mbbschem07,

Since enantiomers have identical physical properties, their separation requires that either the solid phase is chiral, or you have chiral additive in the liquid phase that interact with you molecules of interest.

Please click the link to this very recent review:

Wistuba D. (2010) Chiral silica-based monoliths in chromatography and capillary electrochromatography  Journal of Chromatography A 1217: 941-952.
The whole issue is dedicated to chiral separations, so you might get more ideas from other articles.

Cheers,