I want help to separate the above describe. I got a Na-salt of p-cresol and told by R&D tell us the unreacted p-cresol.
I did the derivatization with chloroacetyl chloride and inject on HPLC as per attachment, I got around 16% p-cresol in the mixture. It gives me some of doubt.
I want to know from u all that there is another procedure to derivatize and separate these two on RP-HPLC??? If yes please suggest me.
If now then tell me how can I measure the actual concentration of salt???