Thin layer chromatography of lipids

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kipngetich
kipngetich's picture
Thin layer chromatography of lipids

Hi everyone,
Lately i have been separating lipids using TLC, but i have had some problems regarding the separation. the separation is not clear i get a smear and a big spot after visualizing with iodine. my solvent system is Hexane: diethylether:acetic acid in the ratio of 80:20:1. what could be the problem.

Thanks
Kipngetich

Rajeshwari patel
Rajeshwari patel's picture
Hi kipmgetich,

Hi kipmgetich,

I think you should check all your regent first specialy your layer. Second  temprature of your chamber in which you are running your TLC