Thin layer chromatography of lipids

2 posts / 0 new
Last post
kipngetich's picture
Thin layer chromatography of lipids

Hi everyone,
Lately i have been separating lipids using TLC, but i have had some problems regarding the separation. the separation is not clear i get a smear and a big spot after visualizing with iodine. my solvent system is Hexane: diethylether:acetic acid in the ratio of 80:20:1. what could be the problem.


Rajeshwari patel
Rajeshwari patel's picture
Hi kipmgetich,

Hi kipmgetich,

I think you should check all your regent first specialy your layer. Second  temprature of your chamber in which you are running your TLC