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afcollart's picture

I look for an easy method to quantify thiocyanate in urine by HPLC-UV.
Did some one have an idea? What is the spectrum of thiocyanate in UV?

Dr. Analytical
Dr. Analytical's picture
Thiocyanate is highly water

Thiocyanate is highly water soluble so retention in a conventional reversed phase system will be minimal. You will have to find a way to retain a compound with a negative charge.

An ion exchange column is one possibility. There are some "mixed mode" phases from SiELC that may also work.

For detection, you will have to measure a solution in a spectrophotometer to determine the best wavelength to use. Thiocyanate also forms colored complexes with many metals, so you may be able to use a complex for detection and separation.

This could be a difficult project if you need to detect very low levels.

steven's picture
You can check this method at