hello every one
i want method for vitamins extraction from food and human serum
to be determine in HPLC.
Which vitamins do you want to extract?
There are many methods available. Start by looking at the organizations that approve methods - USP, European Pharmacopoeia, Japan Pharmacopoeia, etc. Also, the Association of Official Analytical Chemists (AOAC) has many methods for food, but the extraction procedures will usually be different for each type of food. Some of the methods will be simple, while others may require many steps.
i want sample method to extract vitamine A ,E and folic acid
its my first step in HPLC/UV detector i"ll be gratfull if you guide me to the right direction