Uv-Visible Spectrophotometric methods

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humaira
humaira's picture
Uv-Visible Spectrophotometric methods

Hello,
I need help in developing some new UV-Visible spectrophotometric methods for estimations of some new drugs containing primary ,secondary amines and phenolic hydroxy groups.Can i get the list of reagents which are responsible for color reactions with these groups.
Thanx

otamajakushi
otamajakushi's picture
I think the flow injection

I think the flow injection analysis group can help for your interest.

asoka
asoka's picture
Are the amines aromatic

Are the amines aromatic amines?

humaira
humaira's picture
asoka wrote:Are the amines

asoka wrote:

Are the amines aromatic amines?

Hello,Ya ,they are aromatic primary ,secondary and tertiary amines

Lynn E.
Lynn E.'s picture
You could try HPLC to

You could try HPLC to separate the compounds, then use ninhydrin to derivatize the amino compounds.  If I recall, Pickering had a module to do this with.  It works for primary amines, not sure on the secondary and tertiary ones...

Phenolics themselves will react with 4-amino antipyrene (EPA 420.4 has some more details on the reagents).  Phenols with different substitutions react somewhat differently.  It seems if what you really have is a mix, the first step will be figuring out how to separate the different entities, and go from there.....