Edema

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Platelet Pete
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Edema

Hi, I'm Platelet Pete. I do injury research (burn, ischemia/reperfusion). I would like to find a simple straightforward method to determine pulmonary edema using Evan's blue or fluorescent albumin (low mw and high mw). What I would like is a quick and easy method whereby I inject the dye(s), perfuse to wash out the dyes in the vasculature and then snap freeze the tissue for frozen sections and fluorescence microscopy for the tags. Evan's blue fluoreses red using the FITC channel. Thanks.

Guy Sovak
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Hi Platelet Pete,
I will also try to do some Evans Blue in Nerve tissue and maybe albumin injection.
If you have already some results would be happy if you can share them with us.
Maybe add a protocol of how you do it.
Thanks
Guy

Platelet Pete
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Hi Guy,

Hi Guy,
There are a number of protocols out there. I've read about these methods but haven't done them. I know there is a paper which I can't find that does the Evan's Blue injection - incubation- perfuse-finally snap freeze whole tissues in liquid nitrogen. Then you can section your frozen sections and visualize the edema by fluorescence microscopy. Most of the methods do the Evan's Blue extraction and O.D. measurement. Both have their benefits and pitfalls. Since I am doing histopathology as part of my work, I want to be able to record images of tissue edema. If you find this paper, please share.
Thanks,
Platelet Pete