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jack_prem's picture

Thank you for reading my question.

Can someone explain the purpose of using methylsulfate in the internal solution such as CsMeSO4 or KMeSO4, please?

Thank you

Simon8's picture
frasermoss posted someting

frasermoss posted someting here, that might be usefull:


Posted By frasermoss
on 2/18/2010 9:55 AM   

What is the major counter ion in your pipette solution?
Cl-, Gluconate or MeSO4?
Apparently KMeSO4 in the pipette preserves neuronal excitability more effectively than K-gluconate.

Velumian, A. A., Zhang, L., Pennefather, P., and Carlen, P. L. Reversible inhibition of IK, IAHP, Ih and ICa by internally applied gluconate in rat hippocampal pyramidal neurones. Pflügers Arch. 433: 343-350, 1997

Zhang, L., Weiner, J. L., Valiante, T. A., Velumian, A. A., Watson, P. L., Jahromi, S. S., Schertzer, S., Pennefather, P., and Carlen, P. L. Whole-cell recording of the Ca2+-dependent slow afterhyperpolarization in hippocampal neurones: effects of internally applied anions. Pflügers Arch. 426: 247-253, 1994

Last edited Feb 18, 2010, 11:56 AM by frasermoss"

The FFM's picture
 MeSO4- is an alternative

 MeSO4- is an alternative counter ion to Gluconate when making a Chloride free solution