Osmolarity of ACSF

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vilsy
vilsy's picture
Osmolarity of ACSF

Hi Guys,
I am doing brain slices with mice and off late and my cells have been swelling up...I think the osmolarity of my solution might be wrong...can you guys tell me what is the osmolarity of the in vitro experiments with mice...
The Osmolarity of my solution is 290
Thanks,
Vilay

Fraser Moss
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Typically I've seen values of

Typically I've seen values of 270-300 mOsm/L in the literature
 

vilsy
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Thats what i have seen too...

Thats what i have seen too...
But i cant come up with another reason for the swelling up of cells...Any ideas?
 
Thanks,
Vilay

The FFM
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perhaps the thermocouple in

perhaps the thermocouple in your osmometer is dirty and needs cleaning as well as recalibrating, and so the readout it gices you might not be true.
perform a "clean test" on your osmometer is it has such a function and read the manual for how to clean the thermocouple.
 
 

shuali
shuali's picture
vilsy wrote:

vilsy wrote:

Hi Guys,
I am doing brain slices with mice and off lately my cells have been swelling up...I think the osmolarity of my solution might be wrong...can you guys tell me what is the osmolarity of the in vitro experiments with mice...
The Osmolarity of my solution is 290
Thanks,
Vilay

Hi,
I'm new here. I also record from brain slices, and the osmolarity of my aCSF is around 310 mOsm - depends on my internal solution...

vilsy
vilsy's picture
shuali wrote:

shuali wrote:

vilsy wrote:
Hi Guys,
I am doing brain slices with mice and off lately my cells have been swelling up...I think the osmolarity of my solution might be wrong...can you guys tell me what is the osmolarity of the in vitro experiments with mice...
The Osmolarity of my solution is 290
Thanks,
Vilay

Hi,
I'm new here. I also record from brain slices, and the osmolarity of my aCSF is around 310 mOsm - depends on my internal solution...

 
Can you elaborate as to how it depends on the internal solution?

shuali
shuali's picture
vilsy wrote:

vilsy wrote:

shuali wrote:
vilsy wrote:
Hi Guys,
I am doing brain slices with mice and off lately my cells have been swelling up...I think the osmolarity of my solution might be wrong...can you guys tell me what is the osmolarity of the in vitro experiments with mice...
The Osmolarity of my solution is 290
Thanks,
Vilay

Hi,
I'm new here. I also record from brain slices, and the osmolarity of my aCSF is around 310 mOsm - depends on my internal solution...

 
Can you elaborate as to how it depends on the internal solution?

Yes, As I was teach the osmolarity of the internal solution should be 90 percent of that of the external solution. In the beginning I prepare the internal solution (generally around 285mOsm), according to the requirements of the current I am going to record, and then I adjust the osmolarity of the aCSF.
 

Dpk
Dpk's picture
hi.. am working on brain

hi.. am working on brain slices too. but the slices tend to fold inwards after  1-2 hrs.  Could this be because of  the osmolarity of my acsf??  The osmolarity is usually 310 -320.  I ve not been able to record field potentials consistently too.  Does the incubation chamber need to be kept closed?   could this change osmolarity?? i use 10 mM glucose.

Rajiv Dua
Rajiv Dua's picture
Dear Vilsy...

Dear Vilsy...
                     Normally  280-310 mOsm/L in the various articles....u may check ur osmometer for that if it is working fine...nor calibrate it

with regards,

Rajiv

Residude
Residude's picture
 Cells can also swell due to

 Cells can also swell due to general cell death, not necessarily problems with osmolarity