Hello!
We do extracellular field recording in mouse hippocampi and I have a question about inducing LTP via Theta Burst Stimulation. All my work is usually done with 2 trains of 100Hz (High Frequency Stimulation) but I'm doing an experiment with Theta Burst Stimulation.
I think someone messed up the Clampex program because if I'm right, the slice gets stimulated with 4 pulses at 200Hz instead of 100Hz. Can someone look at this to tell me if it's correct? I have done a lot of ephys on this protocol already and would hate to redo it because it isn't stimulating voltage-gated Calcium channels.
Thanks!
I highlighted things that may be of interest.
Clampex protocol:
Created by: Clampex 10.2.0.18
Acquisition mode: episodic stimulation.
Axon binary file (integer)
40000 samples in this file.
Comment: n/a
Experiment type: other.
Input signal: IN 0 (#0). Telegraphs disabled.
Input signal: IN 1 (#1). Telegraphs disabled.
Input signal: IN 2 (#2). Telegraphs disabled.
Input signal: IN 3 (#3). Telegraphs disabled.
Trial length: 6 runs; 1 sweep of 1000.0 ms (10000 samples per channel).
10.000 s between run starts.
Minimum time between sweep starts.
First sampling rate: 10.00 kHz (interval 100.00 µs).
Second sampling rate: 5.00 kHz (interval 200.00 µs).
Input signals: IN 0 (#0), IN 1 (#1), IN 2 (#2)
and IN 3 (#3).
Signal Cmd 0 (#0) holding level: 0.00 mV.
Signal Cmd 1 (#1) holding level: 0.00 mV.
Signal Cmd 2 (#2) holding level: 0.00 mV.
Signal Cmd 3 (#3) holding level: 0.00 mV.
Analog Output #0
Waveform:
EPOCH A B C D E F G H I J
Type Off Train Step Train Step Train Step Train Step Train
Sample rate Fast Fast Fast Fast Fast Fast Fast Fast Fast Fast
First level (mV) 0 5.0 0 5.0 0 5.0 0 5.0 0 5.0
Delta level (mV) 0 0 0 0 0 0 0 0 0 0
First duration (samples) 0 200 2000 200 2000 200 2000 200 2000 200
Delta duration (samples) 0 0 0 0 0 0 0 0 0 0
First duration (ms) 0.0 20.0 200.0 20.0 200.0 20.0 200.0 20.0 200.0 20.0
Delta duration (ms) 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0
Train Period (samples) 0 50 0 50 0 50 0 50 0 50
Pulse Width (samples) 0 1 0 1 0 1 0 1 0 1
Train Rate (Hz) 0.00 200.00 0.00 200.00 0.00 200.00 0.00 200.00 0.00 200.00
Pulse Width (ms) 0.0 0.1 0.0 0.1 0.0 0.1 0.0 0.1 0.0 0.1
Intersweep holding: same as for signal Cmd 0 (#0).
Digitial Out pattern: disabled.
Pre-sweep train: disabled.
Leak subtraction: disabled.
User list: disabled.