Theta burst stimulation protocol question (Clampex)

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mousy49
mousy49's picture
Theta burst stimulation protocol question (Clampex)

Hello!
We do extracellular field recording in mouse hippocampi and I have a question about inducing LTP via Theta Burst Stimulation. All my work is usually done with 2 trains of 100Hz (High Frequency Stimulation) but I'm doing an experiment with Theta Burst Stimulation.
I think someone messed up the Clampex program because if I'm right, the slice gets stimulated with 4 pulses at 200Hz instead of 100Hz. Can someone look at this to tell me if it's correct? I have done a lot of ephys on this protocol already and would hate to redo it because it isn't stimulating voltage-gated Calcium channels.
Thanks!
I highlighted things that may be of interest.
Clampex protocol:

Created by: Clampex 10.2.0.18
Acquisition mode: episodic stimulation.
Axon binary file (integer)
40000 samples in this file.
Comment: n/a

Experiment type: other.

Input signal: IN 0 (#0).    Telegraphs disabled.
Input signal: IN 1 (#1).    Telegraphs disabled.
Input signal: IN 2 (#2).    Telegraphs disabled.
Input signal: IN 3 (#3).    Telegraphs disabled.
Trial length: 6 runs; 1 sweep of 1000.0 ms (10000 samples per channel).
10.000 s between run starts.
Minimum time between sweep starts.
First sampling rate:  10.00 kHz (interval 100.00 µs).
Second sampling rate: 5.00 kHz (interval 200.00 µs).
Input signals: IN 0 (#0), IN 1 (#1), IN 2 (#2)
and IN 3 (#3).

Signal Cmd 0 (#0) holding level: 0.00 mV.
Signal Cmd 1 (#1) holding level: 0.00 mV.
Signal Cmd 2 (#2) holding level: 0.00 mV.
Signal Cmd 3 (#3) holding level: 0.00 mV.

Analog Output #0
Waveform:
EPOCH                                A      B           C         D        E        F       G           H         I      J
Type                                    Off     Train   Step   Train  Step  Train   Step   Train   Step     Train 
Sample rate                    Fast    Fast    Fast   Fast   Fast    Fast   Fast     Fast    Fast     Fast  
First level (mV)                   0      5.0        0        5.0        0        5.0        0         5.0       0         5.0   
Delta level (mV)                 0      0            0          0          0         0          0          0         0           0     
First duration (samples)  0      200     2000    200    2000   200    2000    200    2000     200   
Delta duration (samples) 0      0           0           0         0          0          0          0          0          0     
First duration (ms)            0.0    20.0   200.0   20.0   200.0  20.0   200.0   20.0    200.0   20.0  
Delta duration (ms)          0.0    0.0       0.0       0.0      0.0     0.0        0.0       0.0       0.0       0.0   
Train Period (samples)    0      50          0         50        0        50          0         50         0         50    
Pulse Width (samples)    0      1             0          1         0          1           0          1          0          1     
Train Rate (Hz)                0.00  200.00  0.00   200.00 0.00   200.00 0.00   200.00 0.00   200.00
Pulse Width (ms)            0.0     0.1        0.0       0.1       0.0      0.1        0.0       0.1      0.0       0.1   
Intersweep holding: same as for signal Cmd 0 (#0).

Digitial Out pattern: disabled.
Pre-sweep train: disabled.
Leak subtraction: disabled.
User list: disabled.