unable to convert AxographX file for analysis in minianalysis

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jilli
jilli's picture
unable to convert AxographX file for analysis in minianalysis

Hi,

I am experiencing a really annoying problem. I am trying to analyze a file captured in
Axograph X in minianalysis.

I saved the file in ABF for it to be compatible with Minianalysis. When I tried to open
the file in minianalysis I got the message that the program takes only integer format-
ABF.

I converted floating-point ABF to integer format ABF using clampfit.

Now I am able to open the file in minianalysis but I cant magnify more than .1nA. If I
try to increase the gain further the program shows an error message

"Run time error '380' Invalid property value" and Minianalysis program terminates.

I am trying to analyze miniature currents and need a better resolution than this. Can anyone
please help.

Thanks

The FFM
The FFM's picture
During the file conversion in

During the file conversion in Clampfit there may be a problem in specifying the numeric precision of the ABF file for use and rescaling in Minialaysis (a program that I have not used by the way).

Not sure how you fix that  - but simple question - Why not do the analysis in Clampfit if you have it?

Which version of Clampfit are you using? 

jilli
jilli's picture
Thanks so much for your reply

Thanks so much for your reply. I wrote to Minianalysis people again and still no reply!

I have Clampfit 10.2.0.12.

I played around a little bit with Clampfit's event detection parameters. Seems like its not that bad. Have you done IPSC or EPSC analysis in Clampfit?

Do you have any specific suggestions for someone starting from the beginning?

jilli
jilli's picture
It seems that minianalysis

It seems that minianalysis can show voltage or current values only in a certain range, my ABF files were out of range. Changing the units of the file using ABF utility solved the problem.

The FFM
The FFM's picture
I'm glad you've fixed the

I'm glad you've fixed the problem and thanks for letting us know the outcome of your trails.

Would you mind leaving a post with a step by step guide how to rescale your data for analysis using the ABF utility?

that info might be very useful for anyone else having similar problems.
Thanks