Ab / peptide immobilization

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samm
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Ab / peptide immobilization

Has anybody used the Pierce (Now Thermo scientific) Sulfolink Ab immobilization kits? Do those work better for small peptides (e.g. ones used for Ab affinity purification) than the carboxyl-crosslinking systems? And finally, does the cysteine based background quenching affect protein binding properties (again, for e.g Ab-peptide interactions)?

samm
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Just for the record, the

Just for the record, the SulfoLink kit worked pretty well, with nearly 65% of the loaded peptide being immobilized. Between 50-80% of non-specific binding (depending on cell type: the original Ab showed no difference, now I see distinct expression differences between neutrophils and monocytes) was eliminated, based on FC assays with different dilutions/blocking peptide.

Mangarita
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samm wrote:Has anybody used

samm wrote:

Has anybody used the Pierce (Now Thermo scientific) Sulfolink Ab immobilization kits? Do those work better for small peptides (e.g. ones used for Ab affinity purification) than the carboxyl-crosslinking systems? And finally, does the cysteine based background quenching affect protein binding properties (again, for e.g Ab-peptide interactions)?

I have preowned products that might be able to help
Michael