Anti HSP90

4 posts / 0 new
Last post
Guy Sovak
Guy Sovak's picture
Anti HSP90

Hi,
I am getting lots of problems with anti HSP90.
Would like to get some idea of antibody that you are using.
I am using Stressgen , its not working.

Guy Sovak
Guy Sovak's picture
Hi All,

Hi All,
I found a solution,
So I am using an Anti Rabbit anti HSP90 Ab.
Worked great.
In my case I incubated it over night.
Guy

StephenJones1
StephenJones1's picture
Hi everyone,

Hi everyone,

This antibody is reactive with both the constitutive and the inducible forms of HSP90. It does not bind to the native form.
HSP70 and HSP90 are molecular chaperones expressed constitutively under normal conditions to maintain protein homeostasis and are induced upon environmental stress. Both HSP70 and HSP90 are able to interact with unfolded proteins to prevent irreversible aggregation and catalyze the refolding of their substrates in an ATP- and co-chaperone-dependent manner. Polyclonal antibodies are produced by immunizing animals with synthetic peptides surrounding Glu289 of human HSP90. Antibodies are purified by protein A and peptide affinity chromatography. HSP90 Antibody detects endogenous levels of total HSP90 protein. This antibody does not cross-react with other HSPs. Munohistochemical analysis of paraffin-embedded human colon carcinoma, using HSP90 (E289) Antibody. Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using HSP90 (E289) Antibody in the presence of control peptide (left) or antigen-specific peptide (right). Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using HSP90 (E289) Antibody.

Thanks and Regards,
Stephen Jones

Robertwe
Robertwe's picture
View the presentation - B

View the presentation - B-cell epitope prediction for immunity mediated by antipeptide antibodies: evolving translational perspectives on October 18, 2012 for free and in real time. Check details
1">