Vote on best antibody for FLAG tag

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ZhanglinShen
ZhanglinShen's picture
Vote on best antibody for FLAG tag

 Hello everyone,

I would like to start a discussion/vote on which antibody (polyclonal or monoclonal) works best for detecting FLAG fusion protein in Western blot.

I have used the M2 from Sigma
http://www.sigmaaldrich.com/catalog/ProductDetail.do?D7=0&N5=SEARCH_CONCAT_PNO|BRAND_KEY&N4=A2220|SIGMA&N25=0&QS=ON&F=SPEC

and also the rabbit anti ECS from Dualsystems Biotech: http://www.dualsystems.com/products/antibodies.html

Both antibodies detect my fusion proteins (some of which are up to 250 kDa). The M2 is a bit cleaner,  but seems to be less sensitive (?). Also, the M2 is quite expensive and I wonder if there are any other options out there?

Thanks for your ideas!