antibody works fine in DAB staining but no signal with fluorescent staining

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gswetha
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antibody works fine in DAB staining but no signal with fluorescent staining

hi,
the antibody i am using for staining mouse brain sections (cryosections) works wonderfully with DAB, however i am not getting any signal for fluorescence. my steps are same for both the stainings except for quenching and signal amplification steps are present for DAB staining but not in fluorescence. Is it that some antibodies which work for DAB might not work for IF?

please help