cFos IHC in frozen brain tissue?

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Foteini Delis
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cFos IHC in frozen brain tissue?

Hi,
I have a bunch of frozen mouse brains stored at -80C that will be sectioned at 15um and thaw mounted on slides for receptor autoradiography.

Does anyone know if I will be able to get a good immunohistochemical reaction for cFos if I post-fix the sections?
Does anyone know which is the best post-fix solution for cFos? 4% PFA? Methanol? Something else?
And which is the most used and reliable antibody?

Thx!
Foteini