Co-immunoprecipitation from frozen lysate.

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JayC
JayC's picture
Co-immunoprecipitation from frozen lysate.

Hi everyone,

I'm quite new to CO-IP studies and was wondering if you could help me with a question. I guess it's better to peform a CO-IP from fresh lysate, but does anyone know whether freezing your lysate (and thawing) before immunoprecipitation would affect protein-protein interactions?

Many thanks,

Jason