IgM isolation

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Dr.Suja's picture
IgM isolation

Hi, I need protocols, articles or references regarding IgM isolation from serum using protein A sepharose,,using an high ionic strength, to increase the binding affinity of Protien a to IgM. Please help.

jangy's picture
Just do a search for "Protein

Just do a search for "Protein A Binding Buffer". If that doesn't help, let me know, I can help. Salt is what drives the binding. I think the pH was at about 9 as well.