I was wondering on average, how long does it take to stain about 15 brain slices using antibodies that detect ChAT, with a secondary antibody that is detected by AB complex (I think that's the name). The brain slices I'm using are not on 'slides', but are free floating in a staining well.
As it stands now, the protocol I'm using takes a very long while and I was wondering if the protocol I'm using is just inefficient, or if it's just average.
I'm going to admit that I'm new at IHC, and I have only tried staining a few times so plesae excuse me if I do not use the proper terms to describe my problem!
It's nice to know that there are scientists out there that actually like to chat about science! =)!