Length of time to complete a IHC staining procedure.

1 post / 0 new
The undergrad
The undergrad's picture
Length of time to complete a IHC staining procedure.

I was wondering on average, how long does it take to stain about 15 brain slices using antibodies that detect ChAT, with a secondary antibody that is detected by AB complex (I think that's the name). The brain slices I'm using are not on 'slides', but are free floating in a staining well.

As it stands now, the protocol I'm using takes a very long while and I was wondering if the protocol I'm using is just inefficient, or if it's just average.

I'm going to admit that I'm new at IHC, and I have only tried staining a few times so plesae excuse me if I do not use the proper terms to describe my problem!

It's nice to know that there are scientists out there that actually like to chat about science! =)!