Looking for help with hemagglutination assay

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nehp
nehp's picture
Looking for help with hemagglutination assay

Hello everybody!

While searching for step by step guides to hemagglutination assays, I found this forum. This is my first post here! My question might sound strange to you:

I am a former aerospace engineer now working in the field of laboratory robotics (i.e. I have no clue about experimentation and antibodies etc). We are looking for applications which can be conducted by robots, and are trying to automate hemagglutination assays at the moment.

A book is ordered, but at the moment I am trying to find an assay kit for hemagglutination tests. I am not able to decipher all the antibodies and terms. Do you know of a Kit which is, for example, used for education which I can buy?

My second question is: A biologist told me that the last step in the assay is to turn the plate to detect the agglutination in the well with a given dilution. Why do you need to turn the plate? Pictures show that there is either a transparent but homogenous color or a red dot in the center and transparent liquid around. Why do biologists turn the plate?

Are there any step-by-step guides to that assay online? (Otherwise I hope for the ordered book)...

Last: I am not even sure if this is the right place to post my question?

It is quite difficult to get started with such topics as an engineer, therefore I look forward to your answers. Thank you very much in advance for your help!

kai

Sami Tuomivaara
Sami Tuomivaara's picture
nehp,

nehp,

This is indeed the right place to post your questions about bioscience research, welcome to the forum.

To your first question: Please take a look at this, or this... You can probably find more by googling commercial hemagglutination kits.

To your second question: Sometimes hemagglutination test result is hard to interpret because there are intermediate forms (partial hemagglutination) between the full hemagglutination (homogenous "mat") and no hemagglutination at all (red dot is called "button'). The tilting of the plate is to ascertain negative result, if there is no agglutination, the cells will flow freely to form a teardrop shaped structure upon tilting the plate at appr. 45 degree angle for a 30 seconds or so. If there is positive or partial hemagglutination, terdrop will not form...

I could also suggest to use ELISA protocols to test your robot.... I think ELISAs are very suitable for large scale experimentation that robots can do...

Cheers,