low-level periplasmic expression scFvs

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dog1985
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low-level periplasmic expression scFvs

hi, I've examed several conditions (temperature, time, concentration of inducer) to induce scFvs. However, it seems to express low-level (2mg/L) in experiments. The procedure was  based on Barbas manual. Should we try other details?

vector:pComb3X
bateria strin: HB2151
medium: SB
temperature:30 and 37 (o/n)
induction condition: IPTG 0.1mM-1mM (final) at A600=0.6-0.7