I'm facing a well known problem with phalloidin: it doesn't work with a fixation in Methanol.
My issue is that the only antibodies i have found for my protein of interest and working in immunocytochemistery are working with the fixation in Methanol.
Somebody told me that i should try to start with a PAF fixation, and then 5 minutes with Methanol.
What do you think about this? I'm working on mouse primary cortical neurons.
Thank you very much,