recombinant monoclonal antibody

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realistic
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recombinant monoclonal antibody

Analytical methods to quantitate and assess charge variants in recombinant monoclonal antibodies and their structural characterisation.

realistic
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Mol weight of Mab is 150KDa.

Mol weight of Mab is 150KDa. But on a 10% SDS Non-reducing PAGE it aligns above the 200KDa marker. But after reduction has two bands of approximately 50KDa and 25KDa. What could be the reason?

samm
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Glycosylation and marker

Glycosylation and marker variability are magnified in non-reducing native conditions - a more stringent technique will show ~155-165 kD for IgG isotypes.
2x55kD+2x25kD in reducing SDS gels.