subclass of mouse IgG

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wangwf1976
wangwf1976's picture
subclass of mouse IgG

I want toknow how to get the antiserum to subclasses of mouse IgG.Please tell me ! Thanks

Arvind Singh Pundir
Arvind Singh Pundir's picture
Hi wangwf1976,

Hi wangwf1976,

hope this papers help you in your querry :
The Four Subclasses of IgG Can Be Isolated from Mouse Serum by using Protein A-Sepharose www3.interscience.wiley.com/cgi-bin/fulltext/119576747/PDFSTART

heehawmcduff
heehawmcduff's picture
If you want to purchase them

If you want to purchase them commercially then companies like MP Biomedicals can supply them for you
(http://www.mpbio.com/advanced_search_result.php?is_quicksearch=true&keywords=antiserum&product_name_filter=on&catalog_number_filter=on)

wangwf1976
wangwf1976's picture
yeah,I know protein A can

yeah,I know protein A can isolate the four subclasses of mouse IgG.but I am not sour how can I get the specific antiserum against each of them.Using purified IgG to imuunize
rabbit or one of the fraction.

Abhishek Singh
Abhishek Singh's picture
posssibly by immunizing other

posssibly by immunizing other host species with mouse serum and then doing affinity chromatography with desired subclasses IgG.