Western Blot on a 500kDa Protein

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hulkster
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Western Blot on a 500kDa Protein

Hi everyone,

I am currently trying to resolve a 500kDa protein, but to no avail. I am using a 7.5% polyacrylamide gel in a 1X Tris glycine buffer.

Moreover, I can't seem to find a protein ladder suitable for a molecular weight of this size. NuPage makes one called the HiMark, but its for tris-acetate gels (ones only they supply)

Any suggestions??

Thanks