Commercial ELISA

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Nardine
Nardine's picture
Commercial ELISA

Hi everybody...
Did any one ever tried to use a commercial human protein assay to quantify a recombinant protein????
and If so did it work fine????

Hatuey
Hatuey's picture
You have to test it. It can

You have to test it. It can or it can not work depending on what epitope of the protein the antibody binds and the structure of the conjugate in the ELISA kit , some kits are developed using recombinant proteins so it could work, but it is not certain.