ELISA for alpha-synuclein

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marijke
marijke's picture
ELISA for alpha-synuclein

I am trying to optimise our ELISA method for alpha-synuclein. We have problems with the standard, which does not seem to stay stable.
Does anybody have an idea what to do about that? Thanks in advance!

Hatuey
Hatuey's picture
What do you mean by "stable"?

What do you mean by "stable"? if is is a reproducibility issue try to improve your pipetting, increase the number of reps, determine if the position of the sample in the plate affects the results in which case you need to coat new plates, try using multichannel pipettes, sometimes the time between standards and sample aliquoting is critical, be careful with mixing all reagents well in a shaker or by hand