I have two questions:
1. In my Elisa results, I have 4 standard calibrators and 1 negative standard (conc= 0 IU/ml).Is it possible to use 4PL or 5PL equations for only 5 data points( 5 standards)?
2. In Elisa test instruction that the company gave me, it is noted that the calibration curve should be a linear plot. But my results can be better explained by non linear curves. Am I allowed to disregard test instruction in this case?
Any help would be appreciated.