Much worried about ELISA !!!!

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Tohed's picture
Much worried about ELISA !!!!

Hi all

I've got ELISA kit for Beta lactoglobulin (it is a milk protein). Company provided me pure beta  lactoglobulin (standard), Coating Antibody and HRP conjugated detecting antibody. I have tried several times but I did not get good standard curve which is expected according to the protocol  provided by the company.