Problem in ELISA

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Tohed
Tohed's picture
Problem in ELISA

Hi all

I've got ELISA kit for Beta lactoglobulin (it is a milk protein). Company provided me pure beta  lactoglobulin (standard), Coating Antibody and HRP conjugated detecting antibody. I have tried several times but I did not get good standard curve which is expected according to the protocol  provided by the company.

Is there anybody who can help me please!!!!

Sami Tuomivaara
Sami Tuomivaara's picture
Tohed,

Tohed,

You need to be more specific about your problem. Can you please post your exact protocol, as well as your data here so we can make suggestions. I've done my share of ELISAs, but with the little information you provided, there is a million things that can be wrong. Your protocol and data could help to narrow down the problem.

Cheers,

Tohed
Tohed's picture
Hi Suola

Hi Suola

Thank you for your kind response. I am posting  the company protocol as well as some data that I have got. I am worried that the my  standard curve does not match with that of protocol.

I am looking for your kind reply !!

Wishing the best for you

Tohed