Total protein increases as the phoso increases?

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Jason Carlesso
Jason Carlesso's picture
Total protein increases as the phoso increases?

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Lets say your measuring a protein like pSTAT3 and Total STAT3 in a dose response experiment..….the total STAT3 increases as the phospho STAT3 level increases……how can this be explained?
 
 
Could it be that there is more of the actual STAT3 being expressed? Less ubiquitination maybe?

Cheers

Jason

Chin Fen Teo
Chin Fen Teo's picture
 Hi JasonStein,

 Hi JasonStein,

I think you need to take consideration of many factors. First comes to mind- how long treatment is that? If it is done in a relatively short time window, I doubt that is the case. But, if it is a very long treatment, it may be real for sure.

From the technical stand point, how did you perform these 2 westerns- Same membrane, first probed with pSTAT Ab, stripped then reprobed with STAT Ab, or 2 independent blots?

Also, how many times have you obtained such result? I usually won't jump to any conclusion without multiple replications with this kind of experiment...

Good luck.