I have been looking the past few days for antibodies to recognize the native PrPc and PrPsc of mouse.
So far, I think I have found some good candidates, but I am wondering if anyone here is a prion specialist and might be able to help me out with options.
I need an Ab that will recognize both PrPc and PrPsc in their native states - without denaturing or Proteinase
Also, an Ab that will recognize ONY PrPc in int native state.
And an Ab that will recognize ONY PrPsc in int native state.
I have found the following Abs:
R2 x x
POM2 x x
3F4 x (will not detect mouse PrP)
FH11 x x (not sure?)
ICSM35 x x
ICSM18 x x (weak)
I am not sure if all of these will detect the mouse PrP, and most of the useful ones I don't know where I could get them... Any ideas or knowledge on this topic?
Thanks very much!