western blotting

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maheswary's picture
western blotting

In western blotting first we do sds page na that time all the proteins will be linearised..
But after that wen we MAbs (which can recognise conformational and linear epitopes)to identify  binds to the linearised protein how? that time as such there is no 
conformational epitope na then how it binds...
wat is the mechanism behind it....
Thank you...