Making 4% PFA with 37% Formaldehyde.

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hopetown
hopetown's picture
Making 4% PFA with 37% Formaldehyde.

 Is it possible to make 4% PFA in PBS from a stock of 37% formaldehyde!  Or is it better to use paraformaldehyde powder? Hope some one can help me!
 

vanshita
vanshita's picture
hello ,

hello ,
            ideally you should use paraformaldehyde powder for 4% PFA. 37% formaldehyde solution contains 10% methanol which is used as stablizer whc limit the polymerization of formaldehyde to paraformaldehyde.As  methanol is present in formaldehyde whc slows down polymerisation thats lead eventually to precipitate paraformaldehyde. thats why 4% PFA formed from formalin or formalehyde also contains 1%methanol.