scFv expression

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Sun Yi
Sun Yi's picture
scFv expression

I've selected the phage display antibody(scFv) library and transduced the phagemid into HB2151 for making scFv. But I can't get the scFv in several conditions, does any one know the optimal expression condition(especially the expression system,the concentration of glucose and IPTG, the tempreture,ect)which was determined empirically?
Thanks in advance!

pwnsingh7979
pwnsingh7979's picture
hello dr. HB2151 system we

hello dr. HB2151 system we are also using for ScFv antibodies production, 2%glucose concentration, 1mM IPTG, 30degree Centigrade temp. is sufficient for the expression. u have to try the induction in some late log phase like whent OD at 600nm reaches up to 1.2-1.4.
with best wishes
pawan