In Vivo Selection

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Jessy332
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In Vivo Selection

Has anyone attempted in vivo selection of phage displayed scFv and if so with what sucsess?

ding
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you mean in a live animal or

you mean in a live animal or on cells in suspension?

Jessy332
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ding wrote:you mean in a live

ding wrote:

you mean in a live animal or on cells in suspension?

on the former

ding
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never tried. sorry.

never tried. sorry.

ding
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on the former

on the former

Anyway you may find some hint in Barbas et al. "Phage Display - a laboratory manual". There's a whole chapter dealing with in vivo selection.

Jessy332
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Anyway you may find some hint

Anyway you may find some hint in Barbas et al. "Phage Display - a laboratory manual". There's a whole chapter dealing with in vivo selection.

Thanks, i have been using Barbas et al as my guide, but this deals more with in vivo peptide library selection rather than antibody display.

omid
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Jessy332 wrote:

Jessy332 wrote:

Anyway you may find some hint in Barbas et al. "Phage Display - a laboratory manual". There's a whole chapter dealing with in vivo selection.

Thanks, i have been using Barbas et al as my guide, but this deals more with in vivo peptide library selection rather than antibody display.

Could you please provide the name of the publisher and year the book is published with the editor/s names.

Jessy332
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Could you please provide the

Could you please provide the name of the publisher and year the book is published with the editor/s names.

Publisher: Cold Spring Harbor Press (2001)
Editors: Carlos Barbas III, Dennis R. Burton. Jamie K Scott and Gregg J Silverman

There is another really good Phage Display book " Phage Display a practical approach" Eds: Tim Clackson and Henry B Lowman (2004)

Hope that helps

ding
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There is another really good

There is another really good Phage Display book " Phage Display a practical approach" Eds: Tim Clackson and Henry B Lowman (2004)

Hope that helps

I never saw Clackson and Lowman but heard it's good. Do you think it better than Barbas et al? and if so why?

Jason King
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Doesn't the animal's immune

Doesn't the animal's immune system wipe out the phages? The innate immune system could also be triggered so that even if T-cell / B-cell deficient animals were used, there would still be phage clearance....wouldn't there?