I am working on a silica-based anion-exchange resin to purify nucleic acids. A couple buffers I am using are containing non-ionic detergents (e.g. Triton X-100). Triton is quite "sticky" to the resin and therefore shows significant tailing, which means, that traces of the surfactant are showing up in the eluate from time to time.
How can I wash off Triton (and other unspecifically bound substances) from the column? We tried alcohols (e.g. buffers with 10% isopropanol or ethanol), but these alcohols are reducing column flow significantly.
I have heard through the grapevine, that amphoteric substances or sugar alcohols (e.g. butanediol, pentanediol, hexanediol, sorbitol, mannitol) may be helpful with this, but I have never seen any of these substances in any protocol....
Is there anybody out there whomay be able to help me with this?