specific activity of alkaline phosphatase

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hunter300
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specific activity of alkaline phosphatase

 Dear All, can u please help me with this question!

Two patients, A and B, have their serum alkaline phosphatase level measured. 
Patient A has an enzyme specific activity which converts 8 µmoles of 4-nitrophenol phosphate substrate/min/100 ml blood and has a 60% haemocrit (this indicates that 60% of the blood volume is made up of cells). 
Patient B: 0.1 ml of serum was used (haemocrit also 60%) and this produced 14 µg of 4-nitrophenol product (molecular weight 139) in 30 minutes under the standard assay conditions. 
Calculations (show your workings) 
What is the value in µmoles of the total amount 4-nitrophenol produced for Patient B. 
What is the value in µmoles/min of the activity of alkaline phosphatase for Patient B 
What is the specific activity of alkaline phosphatase of Patients A and B 

Many thanks xxxx