Which plug-in or what function of image J do people use to quantify their bands from in western blots?
I've been using the "Analyze/Gel" function, but not sure that is actually the correct way to go.
you use a mac or pc?
I use PC
I found out the best way is to use the Ctrl +M "Measure function" for each band and in the measurement options select "Integrated Density"
This has worked very well since I implemented it.
Any one got an old version for an old PC.
The one that I downloaded from the site needs a Mac OSX I got Mac OS IX
you could also consider this alternatives
(no experience with that I must admit)
for the Ctrl + M, you will need first do the color invert
You can check out www.quantifyimage.com if your using windows and need a commercial tool which includes support.
Recently I came throught this post.
You don't need a separate image J plug-in for quantifying your western blot bands. I have also been using same "Analyze/Gel" option and its correct. It does not make much difference whether you are using windows or mac version of image J. I have tried both the ways.